The victims were in stable condition, the authorities said. What led up to the shooting on I-75 near London, Ky., was not immediately clear.
A section of a Kentucky highway was closed for several hours on Saturday night after five people were shot, the authorities said.
What led up to the shooting was not immediately clear. All five shooting victims were in stable condition, said a spokesman for the Laurel County Sheriff’s Office, Deputy Gilbert Acciardo.
The Laurel County Sheriff’s Office said on Facebook that the shooting happened on I-75, which was closed at Exit 49, nine miles north of London, Ky. It said just after 6:30 p.m. that the highway was closed “due to an active shooter situation,” but did not elaborate.
Randall Weddle, the mayor of London, said in a Facebook video said the authorities were searching for a “suspect or suspects” in “rugged terrain” in the northern part of Laurel County.
Deputy Acciardo said helicopters and infrared scanners were being used to search for the gunman in the woods.
The London Police Department said on Saturday night that a person of interest had been identified and asked the public for any information about his whereabouts. The city of London is about 90 miles south of Lexington.
Just after 9:20 p.m., the Laurel County Sheriff’s Office said on Facebook that while I-75 had reopened, the search for the suspect would continue.
Angel Jarrett was working at the 49er Truck Stop when someone told her that shots had been fired nearby.
Eventually, multiple police cars surrounded the truck stop near the exit where the shooting took place and placed the facility on lockdown.
“We’re not allowed to go in or out,” Ms. Jarrett said. “It’s a little panicky but we’re OK. They’re surrounding us, the cops are.”
Saint Joseph London, a hospital in London that is a part of CHI Saint Joseph Health, said that it had “received multiple patients and is treating them for minor injuries.”
Two patients were being treated at the University of Kentucky’s Albert B. Chandler Hospital, a spokeswoman said. Their conditions were unknown.
The Bureau of Alcohol, Tobacco, Firearms and Explosives said on social media that it was sending agents from its Louisville office to help the State Police and local authorities “with a critical incident” near Interstate 75 in Laurel County.
Yan Zhuang contributed reporting.
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